A simple and effective approach for treatment of situs tumor and metastasis：to promote intratumor pus formation

Abstract

Purpose: The recent emergence of the tumor microenvironment as the critical determinant in cancer outcome opens a new routes to fight cancer, however, the clinical results of targeting microenvironment for treating human cancer have not met expectations. Our purpose is to investigate how to target microenvironment for treatment of situs tumor and metastasis.

Methods : We suppose that tumor is a robber from times of anarchy and disorder and can be eradicated in flourishing age. We also suppose that carcinogenesis is largely attributed to physically weak that cann’t get rid of ulcer by pus formation. In vivo，the subcutaneous implant model and pulmonary metastasis model of lewis lung cancer were established. Tumor bearing mice were taken water decoction of Astragalus mongholicus(huangqi) and Spina Gleditsiae (zaojiaoci) by intragastric administration b.i.d for ten weeks， and the influences of Astragalus mongholicus and Spina Gleditsiae  on tumor progression were evaluated by body temperature，blood oxygen saturation，red cell ATPase，blood  rheology，intratumor hypoxia，capillary permeability， matrix metalloproteinase (MMPs) and intratumor pus formation. 　

Results：We found that both of Astragalus mongholicus and Spina Gleditsiae could keep body temperature，blood oxygen saturation，red cell ATPase and blood rheology，and improve intratumor hypoxia，capillary permeability and MMPs in tumor bearing mice，which led to slower tumor growth and less metastasis. Astragalus mongholicus could remove body poison and stimulate immune responses, and Spina Gleditsiae  could  promote pus formation and proteolytic enzymes. The combination of  Astragalus mongholicus and Spina Gleditsiae favored the restoration of tumor immune responses and proteolytic activity at the tumor site, which not only result to an increase in aseptic pus formation, but also to a decrease in necrotic tissue accumulation, and finally caused a complete intratumor pus formation and tumor-free survival in lewis lung cancer model.

Conclusion: Although our success in cancer control is limited to body surface tumor, intratumor pus formation is a simple and effective approach for treatment of situs tumor and metastasis.